Phosphorylated heat shock protein 27 improves the bone formation ability of osteoblasts and <scp>bone marrow stem cells</scp> from patients with adolescent idiopathic scoliosis

Abstract Background Adolescent idiopathic scoliosis (AIS) is a scoliotic deformity of unknown etiology that occurs during adolescent development. Abnormal bone metabolism closely related to AIS, but the cause uncertain. Recent studies have shown heat shock protein 27 (HSP27) and its phosphorylation (pHSP27) play important roles in metabolism. However, whether HSP27 pHSP27 are involved abnormal AIS unclear. Methods Osteoblasts (OBs) marrow stem cells (BMSCs) were extracted from facet joints patients controls who underwent posterior spinal fusion surgery. The expression levels pHSP27, as well formation markers OBs controls, examined by quantitative real‐time PCR (qRT–PCR) Western blotting. mineralization ability was analyzed alizarin red staining after osteogenic differentiation. Heat thiolutin used increase OBs, also investigated. In addition, BMSCs investigated treatment. Results Lower impaired differentiation abilities observed than those controls. Thiolutin increased mRNA SPP1 ALPL patients. treatment expression, levels, OCN both Conclusion can further promote Therefore, decreased may be associated with